Influenza M1 Virus-Like Particles Consisting of Toxoplasma gondii Rhoptry Protein 4
The Korean Journal of Parasitology
;
: 143-148, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-50089
ABSTRACT
Toxoplasma gondii infections occur throughout the world, and efforts are needed to develop various vaccine candidates expressing recombinant protein antigens. In this study, influenza matrix protein (M1) virus-like particles (VLPs) consisting of T. gondii rhoptry antigen 4 (ROP4 protein) were generated using baculovirus (rBV) expression system. Recombinant ROP4 protein with influenza M1 were cloned and expressed in rBV. SF9 insect cells were coinfected with recombinant rBVs expressing T. gondii ROP4 and influenza M1. As the results, influenza M1 VLPs showed spherical shapes, and T. gondii ROP4 protein exhibited as spikes on VLP surface under transmission electron microscopy (TEM). The M1 VLPs resemble virions in morphology and size. We found that M1 VLPs reacted with antibody from T. gondii-infected mice by western blot and ELISA. This study demonstrated that T. gondii ROP4 protein can be expressed on the surface of influenza M1 VLPs and the M1 VLPs containing T. gondii ROP4 reacted with T. gondii-infected sera, indicating the possibility that M1 VLPs could be used as a coating antigen for diagnostic and/or vaccine candidate against T. gondii infection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Toxoplasma
/
Vírion
/
Ensaio de Imunoadsorção Enzimática
/
Toxoplasmose
/
Western Blotting
/
Baculoviridae
/
Células Clonais
/
Clonagem de Organismos
/
Microscopia Eletrônica de Transmissão
/
Influenza Humana
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2017
Tipo de documento:
Artigo
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