Study on the Quality Standard of Ganmao Shangfengke Tea / 中国药房

ABSTRACT

OBJECTIVE:To improve the quality standard for Ganmao shangfengke tea. METHODS:TLC was used to identify the Saposhnikovia divaricata and Glycyrrhizae Radix et Rhizoma in the preparation. HPLC was used to determine the content of magnolol and honokiolthe column was Phenomenex Luna C18 with mobile phase of methanol-water(7723,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 294 nm,column temperature was room temperature,and the injection volume 5 μl. RE-SULTSThe TLC spots of S. divaricata and Glycyrrhizae Radix et Rhizoma were clear and well separated. The linear range was 3.13-100.2 μg(r=0.9999) for magnolol and 3.05-97.6 μg(r=0.9999) for honokiol;RSDs of precision,stability and accuracy tests were lower than 2%;recoveries were 98.2%-99.5%(RSD=0.5%,n=6)and 98.6%-99.6%(RSD=0.4%,n=6),respective-ly. CONCLUSIONS:The improved standard can be used for the quality control of Ganmao shangfengke tea.