Inhibitory effects of Sanguisorba officinalis L.on MRSA biofilms formation / 中国生化药物杂志

ABSTRACT

Objective To investigate inhibitory effects of Sanguisorba officinalis L.on biofilms of MRSA41577.Methods Congo red agar method and crystal violet semi-quantitative method were used to detect the biofilms-forming ability of tested strains; TTC assay was used to detect inhibitory effects of Sanguisorba officinalis L.on biofilms formation and mature biofilms of MRSA41577,as well as effects of Sanguisorba officinalis L.in combination with vancomycin on mature biofilms of MRSA41577.Results Sanguisorba officinalis L.showed significant inhibitory both on biofilms formation and mature biofilms, minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of biofilms formation were 1 mg/mL and 8 mg/mL,MIC of mature biofilms was 4 mg/mL.The sensitivity of mature biofilms to vancomycin was greatly increased when Sanguisorba officinalis L.was combined with vancomycin with subinhibitory concentrations.Sanguisorba officinalis L.at 1/4 MIC can inhibit mature biofilms when combined with vancomycin at 4 μg/mL, while vancomycin didn't show inhibitory effects on mature biofilms when concentrations were below 64 μg/mL. Conclusion Sanguisorba officinalis L.has significant inhibitory on biofilms formation, the mechanism may be related to Sanguisorba officinalis L.destroyed biofilms and make vancomycin penetrate into the biofilms to finish the bactericidal activity.