Effect of root canal sealers on biocompatibility of human periodontal ligament cells / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6): 871-877, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-502941
ABSTRACT
Objective:
To compare the effects of three root canal sealers with respect to time on biocom-patibility of human periodontal ligament cells (hPDLCs).The sealers included zinc oxide and eugenol based sealers (ZOE),epoxy resin sealers (ERS)and silicone based sealers (SBS).Methods:
hPDLCs were primarily cultured,with the method combining of tissue explant and enzymatic digestion.The cells were then exposed to different extract fluids(1)ZOE extracted for 24 h group ;(2)ZOE extracted for 1 week group;(3)ZOE extracted for 2 weeks group;(4)ERS extracted after 24 h group;(5)ERS extracted after 1 week group;(6)ERS extracted after 2 weeks group;(7)SBS extracted after 24 h group;(8)SBS extracted after 1 week group;(9)SBS extracted after 2 weeks group;(10)Dulbecco modified Eagle’s me-dium/F12(DMEM/F12)as negative control group.Cell morphology was observed under an inverted mi-croscope.Cell proliferation was measured by methyl-thiazol-diphenyltetrazolium (MTT)assay.ALP assay kit was used for measuring alkaline phosphatase (ALP)activity.Sealers of 2 weeks’setting time were then immersed in an osteogenic medium for examination of mineral nodules and calcium deposits.Re-sultsConsidering the relative growth rate (RGR),ZOE was severely to moderately cytotoxic (RGR13.6% -39.9%),while ERS was slightly or not cytotoxic (RGR87.6% -95.3%).Only SBS did not show any cytotoxicity after setting (RGR91.8% -106.7%).The setting time influenced the cytotoxic-ity of ERS which decreased after 1 week.Considering the ALP activity,there was no difference between SBS group and control group(F =3.397,P =0.053).According to the results of calcium deposits, ZOED562 nm =0.180 ±0.050,ERSD562 nm =2.968 ±0.201,SBSD562 nm =3.623 ±0.039,ControlD562 nm =3.477 ±0.102,the ranking of ALP activity and calcium deposits was as followsZOE
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Peking University(Health Sciences)
Ano de publicação:
2016
Tipo de documento:
Artigo
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