Effect of Vaspin in vitro expressed on insulin signal transduction pathway of GDM / 实用医学杂志

ABSTRACT

Objective To investigate the relationship between Vaspin and GDM insulin resistance. Methods The GDM preadipocyte cells were recoveried, extended and differentiated.Vaspin overexpression carriers were made to transformate, cultivate and extract the plasmid. The fat cells were transfected and extended using 4 overexpression levels (0.0 μg, 1.0 μg, 2.5 μg, 5.0 μg). Q-PCR was used to detect mRNA expression of Vaspin, insulin receptor substrate-1/2 (IRS-1/2), phosphatidy inositol 3 kinase (PI3K (P85a) Western Blot was used to detect protein expression of Vaspin, IRS-1/2, PI3K (P85a) and IRS-1/2 phosphorylation levels, and [3H]-2-deoxidation-D-glucose uptake assay was used to detecte glucose uptake rates. Results (1) According to the Q-PCR and WB results, the constructed Vaspin overexpression carrier was effective; (2) With the Vaspin expression increased, the mRNA and protein expression of IRS-1/2, PI3K (P85a) and IRS-1/2 tyrosine phosphorylation levels had no significant changes;(3) Glucose uptake rate of fat cells had no obvious correlation with Vaspin. Conclusion High expression of Vaspin in GDM serum and omental adipose tissue has no obvious link with the insulin resistance of GDM.