Safety assessment of mammary gland stem cells from normal tissues in breast cancer patients / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 6125-6130, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-503360
ABSTRACT
BACKGROUND:
The cultivation of mammary gland stem cel s is of great significance for the development of mammary gland and breast cancer.OBJECTIVE:
To seek an easy method to isolate and culture mammary gland stem cel in vitro, and verify the safety of cel s.METHODS:
Mammary epithelial cel s were isolated from normal tissues surrounding breast cancer, and CD49f-and EPCAM-positive cel s were sorted using flow cytometry fol owed by surface marker analysis and cel colony formation ability analysis. Afterwards, real-time fluorescent quantitative PCR was used to detect C-erbB-2 and Maspin mRNA expression in mammary gland stem cel s, breast cancer tissues and normal tissues surrounding breast cancer. RESULTS ANDCONCLUSION:
Human mammary gland stem cel s were successful y cultured and highly expressed CD49f and EPCAM, with the presence of mixed colony, pleural epithelial cel colony, and myoepithelial cel colony. c-erbB-2 was lowly expressed while Maspin highly expressed in mammary gland stem cel s. Our experimental findings indicate that the mammary gland stem cel s derived from normal tissue surrounding breast cancer have biological safety.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2016
Tipo de documento:
Artigo
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