Correlation between growth, proliferation and apoptosis of leukemia cell lines K562/AO2 and bone marrow mesenchymal stem cells in children with leukemia / 中国组织工程研究

ABSTRACT

BACKGROUND:Leukemia comes from the damage to bone marrow microenvironment, and changes in bone marrow microenvironment lead to some effects on biological characteristics and behavior of leukemia cel s. OBJECTIVE:To explore the effect of bone marrow mesenchymal stem cel s from children with leukemia on K562/AO2 cel line growth, proliferation and apoptosis. METHODS:K562/AO2 cel s were cultured alone in vitro, or co-cultured with bone marrow mesenchymal stem cel s from leukemia children. The living cel s in the two groups were counted using trypan blue staining, and the cel growth curves were described. PI single staining method was used to detect the cel cycle, and cel apoptosis was detected by V/PI Annexin method in the two groups. RESULTS AND CONCLUSION:After single suspension culture, the cel s showed no marked changes within the first 3 days, but began to proliferate fast at 4 days and reached the proliferation peak at 6 days. Co-cultured cells showed a gentle growth curve with no presence of obvious proliferation peak. Compared with the co-culture group, the proportion of G0/G1 phase cel s was significantly lower, but the proportion of S phase cells was significantly higher in the single culture group (P0.05). All these findings indicate that co-culture with bone marrow mesenchymal stem cel s from leukemia children inhibits the proliferation of K562/AO2 cells through G0/G1 phase arrest, and also resists apoptosis in K562/AO2 cells.