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Screening and identification of immunodominant epitope of human serum albumin / 国际药学研究杂志
Journal of International Pharmaceutical Research ; (6): 1002-1007, 2016.
Artigo em Chinês | WPRIM | ID: wpr-503889
ABSTRACT
Objective To select the immunodominant epitope of human serum albumin(HSA)and provide the basis for set?ting up a special and rapid detecting method of HSA. Methods Bioinformatic method was used to compare protein sequences of hu?man,pig,horse,ox,and ovine,and the immunodominant epitopes of HSA were predicted. The E.coli preferred codons were used to design the DNA sequences of the selected epitopes. The genes of the epitopes were expressed after they were inserted into the PGEX-4T-2 vector. The recombinant antigens were identified and valued by HSA antibody with indirect ELISA. Results The length of the selected epitopes was H1〔126-162 amino acid(aa)〕,H2(314-355aa),and H3(373-424aa)and the relative molecular weight was 3.01×104,3.06×104 and 3.17×104,respectively. The epitope of 373-424 aa were more active and its cross-reactivity IC50 of enzyme-labeled antibody was 1.635 mg/L,which was higher than HSA(P<0.05). Conlusion The immunodominant epitope of HSA is obtained, which is significant for developing a rapid and special reagent of HSA.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Chinês Revista: Journal of International Pharmaceutical Research Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo de rastreamento Idioma: Chinês Revista: Journal of International Pharmaceutical Research Ano de publicação: 2016 Tipo de documento: Artigo