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Over-expression vector construction of human DcR3 gene and its validation / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 1491-1495, 2016.
Artigo em Chinês | WPRIM | ID: wpr-504371
ABSTRACT

Objective:

To construct the human DcR3 expression vector and verify its expression in vitro.

Methods:

915 bp human DcR3 gene CDS was amplified from porcine lung tissues,and was cloned into eukaryotic expression vector pEF1a-IRES-DsRed-Express2 which show red fluorescence. And then pEF1a-IRES-DsRed-Express2-DcR3 was transfected into LX-2 cells by FuGene HD. Expression of mRNA and protein lever of Human DcR3 were detected by RT-PCR and Western blot.

Results:

The levels of DcR3 gene transcription and translation in the hepatic stellate cells were significantly increased after transfection with pEF1a-IRES-DsRed-Ex-press2-DcR3 by RT-PCR and Western blot analysis.

Conclusion:

DcR3 expression vector was successfully constructed and highly expressed in LX-2 cells.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2016 Tipo de documento: Artigo