Content Determination of Berberine Hydrochloride in Fufang Huangge Jiangtang Tablet by UPLC / 中国药房
China Pharmacy
; (12): 3004-3005, 2016.
Article
em Zh
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ABSTRACT
OBJECTIVE:To establish a method for the content determination of berberine hydrochloride in Fufang huangge ji-angtang tablet. METHODS:UPLC was performed on the column of Acquity UPLC BEH C18 with mobile phase of acetoni-trile-0.1% H3PO4(21∶79,V/V)at flow rate of 0.3 ml/min,the detection wavelength was 345 nm,column temperature was 30℃, and the volume injection was 1.0 μl. RESULTS:The linear range of berberine hydrochloride was 0.008 16-0.081 6 μg(r=0.999 9);RSDs of precision,stability and reproducibility were lower than 1%;average recovery was 97.05%-98.96%(RSD=0.66%,n=6). CONCLUSIONS: The method is simple,precise,stablity and reproducible,and can be used for the quality control of Fufang huangge jiangtang tablet.
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Zh
Revista:
China Pharmacy
Ano de publicação:
2016
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Article