Tumor-killing effects of gastric cancer stem cells as antigens to stimulate dendritic cells combined with cytokine-induced killer cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 5345-5350, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-504765
ABSTRACT
BACKGROUND:
As the existence of tumor stem cel s, it is difficult to completely eliminate tumors in clinic.OBJECTIVE:
To explore the tumor-kil ing effect of gastric cancer stem cel s as antigen to stimulate dendritic cel s combined with cytokine-induced kil er cel s.METHODS:
Side population cel s from human gastric cancer cel lines were isolated, and tumor antigen was prepared by freeze thawing method. After coculture with dendritic cel s, dendritic cel s combined with cytokine-induced kil er cel s, gastric cancer cel antigen, and gastric cancer stem cel antigen, kil ing rates of gastric cancer cel s were detected using MTT assay. Expression rate of CD83, a mature dendritic cel surface marker, was also detected. RESULTS ANDCONCLUSION:
The CD83 expression level and kil ing rate of gastric cancer cel s were both significantly lower in the gastric cancer stem cel antigen group than the other groups (both P<0.05). These results indicate that gastric cancer stem cel s as antigen to stimulate dendritic cel s combined with cytokine-induced kil er cel s can promote the proliferation of gastric cancer cel s and elevate the ability to kil ing gastric cancer cel s.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2016
Tipo de documento:
Artigo
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