Expression Profile of Fas-Fas Ligand in Spiral Ganglion Cells During Apoptosis
Clinical and Experimental Otorhinolaryngology
;
: 1-6, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-50497
ABSTRACT
OBJECTIVES:
To examine the expression profile of Fas-Fas ligand (FasL) during glutamate (Glu)-induced spiral ganglion cell (SGC) apoptosis.METHODS:
Cultured SGCs were treated with 10-mM, 25-mM, and 50-mM concentrations of Glu and incubated for 24 or 48 hours. The expression intensity of FasL, Fas, caspase 3, and morphology of single SGC were evaluated using immunofluorescence staining.RESULTS:
In semiquantitative analysis of the Glu-treated SGC, FasL, and caspase 3 expression intensity were increased with concentration- and time-dependent manner. Fas expression intensity did not change with different concentration at 48 hours. In morphologic analysis of the Glu-treated SGC, number of apoptotic cells were increased with concentration- and time-dependent manner.CONCLUSION:
FasL was expressed in apoptotic SGCs, suggesting that the Fas-FasL signaling pathway may be involved in the Glu-induced apoptosis of dissociated SGCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Gânglio Espiral da Cóclea
/
Imunofluorescência
/
Apoptose
/
Ácido Glutâmico
/
Caspase 3
/
Proteína Ligante Fas
Idioma:
Inglês
Revista:
Clinical and Experimental Otorhinolaryngology
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS