Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection
The Korean Journal of Parasitology
;
: 375-380, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-50648
ABSTRACT
Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Imunoensaio
/
Testes Sorológicos
/
Sensibilidade e Especificidade
/
Angiostrongylus cantonensis
/
Diagnóstico
/
Limite de Detecção
/
Angiostrongylus
/
Métodos
/
Anticorpos Monoclonais
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2016
Tipo de documento:
Artigo
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