Development of a Noble Dosimetry Using Metaphase Analysis and Micronuclei Assay of Bone Marrow Cells in Mice / 대한핵의학회잡지
Korean Journal of Nuclear Medicine
;
: 74-81, 2000.
Artigo
em Coreano
| WPRIM
| ID: wpr-50805
ABSTRACT
PURPOSE:
The purpose of this study was to develop in vivo dosimetries using both chromosomal aberrations and micronuclei in mice to assess biological effects of radiations. MATERIALS ANDMETHODS:
Five each mice were irradiated with 0, 1, 2, 3, 4, 5, 10 Gy of Cs-137 gamma-rays. We scored numbers of chromosomal aberrations in metaphase spreads and numbers of micronuclei in bone marrow smears under light microscope, and obtained the dose-response relationships. We also examined the relationship between the two dose-response curves.RESULTS:
The frequency of both chromosomal aberrations and micronuclei increased with dose, in a linear-quadratic manner. The delta, beta, and alpha coefficients were 0.0176, 0.0324, and 0.0567 for metaphase analysis (r=1.0, p<0.001) and 0.0019, 0.0073, and 0.0506 for micronuclei assay (r=1.0, p<0.001). The frequency of chromosomal aberrations and micronuclei in diffirent radiation doses was significantly correlated (r=0.99, p<0.01).CONCLUSION:
In vivo dosimetry using either metaphase analysis or micronucleus assay was feasible in mice. These methods could be useful to evaluate biological effects of radiation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Medula Óssea
/
Células da Medula Óssea
/
Testes para Micronúcleos
/
Aberrações Cromossômicas
/
Metáfase
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Nuclear Medicine
Ano de publicação:
2000
Tipo de documento:
Artigo
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