Detection and identification of high producing huperzine A by an endophytic fungus from Huperzia serrata / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 1112-1116, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-509101
ABSTRACT
Objective To screen endophytic fungal strains isolated from wild Huperzia serrata which can highly produce hu?perzine A. Methods The strain producing huperzine A was identified through thin layer chromatography(TLC)and high-perfor?mance liquid chromatography(HPLC)with authentic huperzine A. The fungus was identified according to its morphological character?istics and nuclear ribosomal DNA ITS sequence. Results The strain was identified as Fusarium oxysporum NSG-1 according to its morphological characteristics and nuclear ribosomal DNA ITS sequence. The amount of huperzine A produced by the fermentation li?quor of this endophytic fungus was quantified to be 1.11 mg/100 ml by HPLC,which was higher than that of previously reported endo?phytic fungi. Conclusion Endophytic fungus producing high huperzine A can be obtained from H. serrata. The production of huper?zine A based on mutagenesis and transformation of the obtained strain may adapt to the industry production.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of International Pharmaceutical Research
Ano de publicação:
2016
Tipo de documento:
Artigo
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