Effect of Yan Kuntai capsule on rat sex hormone level and VEGF bFGF mRNA expression in different stages and premature ovarian failure / 重庆医学

ABSTRACT

Objective To explore the Chinese medicine treatment of QUNTAI capsule in different stages of premature ovarian failure effect.Methods Sixty SD female rats were divided into 6 groupsnormal group,model group,prevention group,intervention group,the treatment group,and Western medicine group.To observe the changes of behavior in rats,enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of follicle stimulating hormone FSH,LH,serum estradiol E2,and anti Mueller hormone AMH.RT-PCR was used to detect ovarian tissue vascular endothelial growth factor (VEGF) and basic effect of fibroblast growth factor (bFGF) mRNA expression.Results The levels of FSH and LH in the normal group and the administration group were significantly lower compare to model group (P＜0.05),and the contents of AMH and E2 were significantly higher compare to model group (P＜0.05).The FSH and LH content in the model group was significantly higher than that of the normal group and each treatment group,E2,AMH content was significantly lower than that of the normal group and each treatment group (P＜0.05);the expression of VEGF andbFGF mRNA in each treatment group and nornial group was significantly higher than that of model group (P＜0.05),Prevention group was higher than intervention group and treat ment group (P＜0.05),there was no Gignificont difference between prevention group and Western medicine group(P＞0.05).Conclusion QUNTAI capsule can increase serum E2 and AMH levels,lower serum FSH,LH levels,improve ovarian sensitivity to sex hormone levels,repair damaged ovarian tissue,promote follicular growth and development,Thereby inhibiting premature follicle depletion.