Expression of Ku70 protein in patients with advanced cervical cancer before and after radiotherapy and its clinical significance / 肿瘤研究与临床

ABSTRACT

Objective To investigate expression change of Ku70 protein before and after radiotherapy in patients with cervical cancer, as well as its relationship with clinical characteristics. Methods Seventy cervical cancer patients who received three dimensional conformal radiotherapy combined with chemotherapy were registered. Specimens tissues were obtained from cervical tumors of irradiation dose of ≤10 Gy and ≥20 Gy group. Immunohistochemistry was used to detect the expression of Ku70 in 70 cases, and its relative expression was analyzed by image software. Results The relative expression levels of Ku70 in cervical cancer patients treated with radiation doses of ≥20 Gy group (1.31 ± 0.83) and ≤10 Gy group (0.71 ± 0.60) were significantly higher than that before radiotherapy (0.50 ± 0.19), and there was a significant difference (t=7.143, P 0.05). The expression of Ku70 in radiotherapy resistant group was significantly higher than that in radiotherapy sensitive group (0.55 ± 0.24 vs. 0.25 ± 0.17, t= 2.979, P< 0.01). Conclusions The expression of Ku70 protein in cervical cancer tissues is increased with the rise of radiotherapy dose. The expression of Ku70 in radiotherapy resistant group is significantly higher than that in radiotherapy sensitive group, indicating that Ku70 protein is correlated with the radiosensitivity, clinical stage, differential degree and tumor diameter.