Content Determination of Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 in Wenyang Zhenshuai Granules by HPLC / 中国中医药信息杂志

ABSTRACT

s Objective To establish an HPLC method to determine the contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Wenyang Zhenshuai Granules.Methods The separation was preformed on Agilent TC-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase consisted of acetonitril-0.05% aqueous solution of phosphoric acid with gradient elution (0–35 min, 19% acetonitrile; 35–58 min, 19%–29% acetonitrile; 58–70 min, 28% acetonitrile; 70–100 min, 29%–40% acetonitrile). The flow rate was 1.0 mL/min; the wavelength of detector was 203 nm; the temperature of column was 35℃.Results The calibration curves showed good linearity in the range of 0.22–2.2 μg (ginsenoside Rg1,r=0.9998), 0.22–2.2 μg (ginsenoside Re,r=0.9998) and 0.26–2.6 μg (ginsenoside Rb1,r=0.9991), respectively. The average recoveries of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 98.04%, 96.58% and 96.75%, respectively.Conclution The method is accurate, hypersensitized and reproducible, which can be applied to the quality control of Wenyang Zhenshuai Granules.