An in vitro study on rabbit osteoarthritis gene therapy with GGCX gene / 重庆医学
Chongqing Medicine
;
(36): 436-438, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-510719
ABSTRACT
Objective To research the effect of GGCX gene on MMP13 in rabbit osteoarthritis cartilage cells and investigate its effect on osteoarthritis cartilage degeneration.Methods Six Japanese big ear rabbits weighted (2.0±0.2)kg were randomly di vided into three groups,each group seted a rabbit as control.Anterior cruciate ligament transection method was used to build osteoarthritis cartilage degeneration model at second,forth,sixth week.Articular cartilage was separated successfully after the model were built,cartilage cells were divided from articular cartilage and cultured in 6-well cell culture plate.Cartilage cells were divided into blank group,negative control group and transfection group.PCR method and Western blot were conducted to detect GGCX and MMP13 expressed at the level of mRNA and protein.Results Compare with blank group and negative control group,the expressin level of GGCX incresed,while the MMP13 expression level dcresed(P<0.05).Conclusion Over expression of GGCX gene can ob viously decrease the expression of MMP13,it provide experimental basis for osteoarthritis of the in vitro gene therapy.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chongqing Medicine
Ano de publicação:
2017
Tipo de documento:
Artigo
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