Analysis of Poria cocos Mycelium Browning by Transcriptome Sequencing / 广州中医药大学学报

ABSTRACT

Objective To study the browning-related genes of Poria cocos mycelia,so as to provide a basis for their further verification and to discuss the browning molecular mechanism of Poria cocos.Methods Four samples of normal and brown Poria cocos mycelia were analyzed by transcriptome sequencing.After aligned with the reference genome sequence of Poria cocos,the expression levels of the genes in different samples were analyzed.Results A total of 12 383 transcripts were identified.Among them,1 017 genes were firstly found,and 260 genes were functionally annotated.Based on the alignment results,336 common differentially-expressed genes overlapped between the normal mycelia and 3 browned mycelia were obtained,and part of them were evaluated.Conclusion Transcriptome sequencing results showed that plenty of differentially-expressed genes were found in brown Poria cocos mycelia,and the difference of the expression levels of some genes was up to several hundred folds or even thousands of folds,whose related-functions were worth of further analysis.