Application of modified agarose electrophoresis for detecting bone alkaline phosphatase isoenzyme in diagnosis of bone metastasisof prostate carcinoma / 国际检验医学杂志

ABSTRACT

Objective To discuss the application of improved agarose electrophoresis for detecting bone alkaline phosphatase(BALP) isoenzymes in the diagnosis of metastasis in prostate carcinoma(PCa).Methods Serum ALP isoenzymes in 50 persons undergoing physical examination and 163 cases of PCa were detected by the improved agarose electrophoresis after treating serum by neuraminidase.Results (1)The resolution of liver and bone ALP by the common agarose gel electrophoresis was lower,and which was increased by the modified agarose electrophoresis.(2)Total ALP and BALP in the PCa group were higher than those in the control group;total ALP and BALP activity in the PCa bone metastasis group were higher than those in the control group and non-metastasis group(P0.05).BALP and total ALP in bone metastasis of prostate carcinoma were higher than no bone metastasis.Conclusion Bone ALP has a certain correlation with PCa bone metastasis,also between bone ALP and bone metastasis of carcinoma.After treating serum by neuraminidase,adopting the improved agarose gel electrophoresis for separating ALP isozymes contributes to the clinical auxiliary diagnosis of PCa bone metastasis.