Bioactive glass modified polyethylene terephthalate promotes bone healing / 中国组织工程研究

ABSTRACT

BACKGROUND:Polyethylene terephthalate (PEA) holds good mechanical properties, but its biocompatibility needs to be improved. OBJECTIVE:To investigate the effect of bioactive glass modified PEA on bone healing. METHODS:In vitro experimentPEA (control group) and bioactive glass modified PEA (experimental group) were respectively co-cultured with MC3T3-E1 cells. The cell morphology was observed at 3 days of culture, and the cell proliferation and alkaline phosphatase activity were detected at 1, 3 and 5 days of culture. In vivo experiment24 New Zealand white rabbits were enrolled, modeled into unilateral knee ligament rupture, and then randomly allotted to two groups. Bioactive glass modified PEA and PEA were implanted into experimental and control groups, respectively. Biomechanics and histological changes were compared between groups at 6 and 12 weeks after transplantation. RESULTS AND CONCLUSION:In vitro experimentthe cell density in the experimental group was significantly higher than that in the control group after 3-day culture (P<0.05);the cell proliferation and alkaline phosphatase activity were significantly higher than those in the control group at 3 and 5 days of culture (P<0.05). In vivo experimentthe maximum axial pull-out strength in the experimental group was significantly greater than that in the control group at 6 and 12 weeks after implantation (P<0.05). In the control group, the defect was obvious and there were a large number of inflammatory cells at 6 weeks after implantation;the defect region became fuzzy, abundant inflammatory cells existed and new bone formed on the surface at 12 weeks after implantation. In the experimental group, there were newly formed bones and trabeculae formed on the interface, the scar tissue became less, and the interface width was narrow at 6 weeks after implantation;numerous new tissues were observed, and only a few inflammatory cells observed in the defect region at 12 weeks after implantation. These results indicate that the modified PEA with bioactive glass possesses good biocompatibility and biomechanics, which can promote bone healing.