Content Determination of Isoniazid in Isoniazid Tablets by Microfluidic Chip with Contactless Conductivi-ty Method / 中国药房
China Pharmacy
;
(12): 1242-1245, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-514982
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of isoniazid in Isoniazid tablet.METHODS:
Mi-crofluidic chip with contactless conductivity detection was adopted. The effects of electrophoresis parameters,such as the variety, concentration,pH,additive types and dosage,injection time and separation voltage on the separation detection were explored. RE-SULTSThe optimized buffer system was 1.86 mmol/L citrate-0.14 mmol/L sodium citrate buffer solution (pH3.0),no additive , the injection time was 20 s and the separation voltage was 2.5 kV. In the optimal conditions,the linear range of isoniazid was 10-200 μg/mL(r=0.9993);the limits of quantification and detection were 8.0 μg/mL and 2.4 μg/mL;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 97.8%-102.9%(RSD=1.9%,n=9).CONCLUSIONS:
The method is rapid and simple,and suitable for the content determination of isoniazid in Isoniazid tablet.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2017
Tipo de documento:
Artigo
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