Identification of a New Adulterated Dye and Study on Detection Method for 9 Orange-yellow Dyes in Typhae Pollen / 中国药房
China Pharmacy
;
(12): 1225-1228, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-514985
ABSTRACT
OBJECTIVE:
To identify a new adulterated dye,and to establish simultaneous detection method for 9 orange-yel-low dyes in Typhae Pollen.METHODS:
TLC and LC-MS were used for the identification of the new adulterated dye;TLC and HPLC method were used for determining 9 orange-yellow dyes. The determination was performed on Welch AQ-C18 column with mobile phase consisting of acetonitrile-0.05 mol/L ammonium acetate solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength were set at 432 nm (lemon yellow,metanil yellow,gold orange O,basic orangeⅡ) and 484 nm (sun-set yellow,acid orange Ⅰ,gold orangeⅡ,orange G,basic orange 21). The column temperature was 35 ℃.The sample size was 10 μL.RESULTS:
TLC spots of auramine O,lemon yellow and new adulterated dye were clear and well separated without in-terference from negative control;new adulterated dye was identified as metanil yellow. TLC spots of lemon yellow,sunset yellow, orange G,acid orangeⅠ,gold orangeⅡ,auramine O,metanil yellow,basic orange 21 and basic orangeⅡwere clear and well sep-arated without interference from negative control. The limits of detection were 0.30,0.20,0.33,0.16,0.19,0.19,0.31,0.26, 0.30 mg/kg.CONCLUSIONS:
The established method is able to detect adulterated dye and orange-yellow dye in Typhae Pollen rap-idly and accurately.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2017
Tipo de documento:
Artigo
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