Establishment of Automation System for Detection of Alcohol in Blood / 法医学杂志

ABSTRACT

Objective T o establish an autom ation system for detection of alcohol content in blood. Meth-ods T he determ ination w as perform ed by autom ated w orkstation of extraction-headspace gas chrom atog-raphy (H S-G C ). T he blood collection w ith negative pressure, sealing tim e of headspace bottle and sam-ple needle w ere checked and optim ized in the abstraction of autom ation system . T he autom atic sam pling w as com pared w ith the m anual sam pling. Results T he quantitative data obtained by the autom ated w ork-station of extraction-H S-G C for alcohol w as stable. T he relative differences of tw o parallel sam ples w ere less than 5%. T he autom ated extraction w as superior to the m anual extraction. A good linear rela-tionship w as obtained at the alcohol concentration range of 0.1-3.0 m g/m L (r≥0.999) w ith good re-peatability. Conclusion T he m ethod is sim ple and quick, w ith m ore standard experim ent process and ac-curate experim ental data. It elim inates the error from the experim enter and has good repeatability, w hich can be applied to the qualitative and quantitative detections of alcohol in blood.