Experimental study of tumor-specific CTL induced by rmIL-18 treated on hepatocellular carcinoma / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 545-548, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-515355
ABSTRACT
Objective:
To research rmIL-18 in vitro culture system CCs induce tumor-specific cytotoxic T lymphocytes CTL and anti-tumor effect in mice.Methods:
Used Stem SepTM immune magnetic cells separation method to culture mouse spleen NK cells,T cells and DCs,established culture systems in vitro;used of different approaches,different doses rmIL-18 to immunize HCC tumor-bearing mice,researched the effect of rmIL-18 on tumor growth rate and survival time.Results:
rmIL-18 could induce and promote tumor-specific CTL-mediated killing effects in vitro culture system;tumor-specific CTL could significantly inhibit tumor growth(P<0.01) of and prolong the survival time of liver cancer tumor-bearing mice(P<0.01),and the effect was increased with rmIL-18 concentration increased(P<0.01),and intratumoral injection was superior to intraperitoneal injection(P<0.01).Conclusion:
rmIL-18 can induce tumor-specific CTL in vitro and play a role in anti-liver cancer in mice.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Immunology
Ano de publicação:
2017
Tipo de documento:
Artigo
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