A study on DNA damage induced by the treatment with the mycotoxins of Alternaria alternata in vitro and the inhibitory effects of R Ⅲ on the DNA damage / 中国病理生理杂志

ABSTRACT

The alkaline elution and fluorometrie DNA assay were adapted to the evaluation of DNA single-strand breaks in the cultured human fetal lung 2BS cell. the DNA damage was induced in vitro by the treatment with Alternariol monomethyl ether (AME) and Alternariol (AOH) which were the main mycotoxins of Alternariol alternata. The results showed that both AME and AOH could induce the DNA single-strand breaks in the cultured human fetal lung 2BS cells and there existed the dose-response association betweenthe fractions of DNA remaining on the filters and the doses. The fractions of DNA remaining on the filters were 0.57?0.04, 0.45?0.02, 0.30?0.02, 0.18?0.01 respetively under the AME concentration of 10, 25, 50, 100?g/ml and were 0.68?0.3, 0.54?0.01, 0.47?0.03, 0.34?0.01 respetively under the AOH concentration of 0.1, 1, 5, 10?g/ml when DNA was eluted for 6hr. It were significantly different from the fractions by solvent control (0.87?0.02) (P