Cytotoxicity to HL-60 cell line and direct viral inactivation of neutrophil defensins / 中国病理生理杂志

ABSTRACT

A number of low molecular weight cationic peptides, named defensins,were purified from neutrophil granuals. Its in vitro cytotoxicity to HL-60 cell line anddirect viral inactivation were examined. The results showed that when incubated with NP-1 and NP-2(100?g/ml) fot 6 hours, more than 60% of HL-60 cells were killed. WhenHerpes simplex virus type I(HSy-I) and influenza virus B strain Beijing 87-1 wereincubated with NP-1(260?g/ml) at 37?C in water bath for 60 min, a marked reduction(98.2% and 90% ) in virus TCID_50 was observed. The results suggested that neutrophilgranulocyte might be able to synthesize peptides which have the cytotoxic and antiviralacitivities.