Antimycobacterial Activities of Dodecyl diaminoethyl glycin hydrolchloride as a Disinfectant / 대한마취과학회지

ABSTRACT

In order to prevent cross-infection, the sterilization of used and/or contaminated anesthetic- apparatus is still a difficult problem in clinical practice, particularly, following anesthesia for known advanced pulmonary tuberculosis cases. Therefore the antibacterisl activities of, an amphoteric detergent, Dodecyl diaminoethyl glycin hydrochloride (Hygien 51) were tested in vitro as a disinfectant against mycobacteria (M. tuberculosis H37 Rv, M. bovis, M. avium, M. tuberculosis H37 Ra, M. phlei, B.C.G.) The antimycobacterial activity tests were performed according to the modified Kolmer method. The testing organisms were cultured in Dubos broth at 37 degrees C, and the detergent, Hygien 51, was diluted with sterile distilled water to 1,3,5 and 10%. One half milliliter of culture was pipetted into each tube containing 4.5 ml of diluted detergent, and at intervals of 3, 15, 30, 60, 120, 180 and 240 minutes, a 4mm loopful of the material was removed from each tube, and transferred to Ogawa media. The tests were carried out at two different temperatures, 20 degrees C and 50 degrees C. The subcultures were incubated at 37 degrees C for more than 8 weeks. The results were obtained as follows 1) At room temperature (20 degrees C), all mycobateria, except M. bovis, were destroyed by treatment- with 10% solution of the detergent for more than 60 minutes. M. bovis was relatively sensitive to the detergent, and was killed by 5% solution for 30 min. and 10% for 15 min., but M. avium. was very resistant, and was not killed by 10% solution for 240 min. 2) By treatment with the detergent at 50 degrees C, the antimycobacterial activities of the detergent were highly increased. All mycobacteria were destroyed by 1% solution for 180 min, and by 10% for less than 30 min at 50 degrees C. In the comparison of the antibacterial activities against mycobacteria and other bacteria, it shows that mycobacteria are generally more resistant to the detergent, Hygien 51.