Detection of micrometastases in the axillary lymph nodes in patients with breast cancer by reverse transcriptase polymerase chain reaction and southern hybridization / 中国普通外科杂志

ABSTRACT

Objective This study was to detect micrometastases in the axillary lymph nodes in primary breast cancer patients and investigate its clinical significance. Methods The cytokeratin 19 gene expression was examined by RT-PCR and Southern hybridization methods. The sensitivity was compared with that of immunohistochemistry(IHC) method.Results The sensitivity was 1∶5?105, 1∶106 and 1∶5?104 by RT-PCR, Southern hybridization and IHC methods,respectively. Thirty-five of 170 routine pathology negative lymph nodes (206%)in 52 primary breast cancer patients contained CK-19 positive signals. Twenty-one out of 33(636%) routine negative lymph nodes were found micrometastases positive in 17 patients who proved to be with lymph node metastases by routine pathologic examination.Eleven out of 35(314%) metastasis negative patients by routine pathology were CK-19 gene positive.Hence the micrometastasis found by our method is an independent parameter.Conclusion RT-PCR method, using CK-19 as a biological marker,was sensitive and specific in detecting micrometastases in primary breast cancer,it could be a new prognostic parameter for breast cancer patients.