Human Keratinocytes Grown in Mitomycin C－treated Human Dermal Fibroblast Feede r Layer / 中华皮肤科杂志

ABSTRACT

0.05).③ HDF feeder layer could improve the efficient growth of primary keratinocyte culture plated at very low seeding densities (0.1? 104 cells/cm2). Conclusions Different allogenic keratinocytes can grow in HDF feeder layers from various passages of the same HDF line. HDF feeder layer is able to improve the seeding efficacy of human keratinocytes, which may be the reason te culture plated at very low seeding