Effect of hepatocyte growth factor on migration and proliferation in cultured retinal pigment epithelial cells / 中华眼底病杂志

ABSTRACT

Objective To investigate the effect of HGF on proliferation and migration in cultured human RPE cells. Methods Human RPE cells cultured in serum-free medium were treated with HGF(1,2,10,50,100 ?g/L), and MTT assay was used to detect the growth of the cells; an in vitro wound healing model was used to count the number of cells that had entered the denudate area in RPE migration treated with HGF (1,2,10,50,100 ?g/L) after 20 h. Results HGF(10,50,100 ?g/L) increased proliferation rates of cultured human RPE (18 2 % to 34 8 %), and at a concentration of 50 ?g/L on day 3 HGF induced the maximal increase of proliferation( P