Effect of antisense oligodeoxynucleotide of human telomerase reverse transcriptase on the telomerase activity of Jurkat cells / 中国病理生理杂志

ABSTRACT

AIM: To observe the effect of antisense oligodeoxynucleotide of human telomerase reverse transcriptase(hTERT) on the telomerase activity in Jurkat cells and its mechanism. METHODS: The telomerase activity was detected by TRAP. hTERT protein and mRNA expressions were detected by flowcytometry and RT-PCR. RESULTS: The absorbency of the cells ( A values) was used to represent the telomerase activity. At 48 h and 72 h, the A values of the Jurkat cells treated with antisense oligodeoxynucleotide of hTERT decreased to 0.351?0.051 and 0.238?0.024, respectively, compared to the A value of 0.492?0.051 in the control cells without treatment ( P