Three dimensional tissue culture in culture medium with rhbFGF / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Artigo
em Chinês
| WPRIM
| ID: wpr-518700
ABSTRACT
AIM:
To investigate the behaviour of 3T3 fibroblast and macrophage co-culture on blood fibrin clot or adipose tissue with recombinant basic fibroblast growth factor (rhbFGF).METHODS:
MTT method, inverted contrast microscopy, Giemsa staining as well as scanning electron microscope were used in the present study.RESULTS:
The effect of rhbFGF on co-culture of 3T3 fibroblast and mouse macrophage on blood fibrin clot in low-serum DMEM with rhbFGF were monitored, and 3T3 fibroblast and macrophage growed well on the blood fibrin blot in low-serum DMEM with rhbFGF.CONCLUSION:
The blood fibrin clot, with its low immunogenicity, could be used as a bionic support for three-dimensional tissue culture, and also a physiological carrier to distribute the growth factor rhbFGF for the cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Artigo
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