Determination of the Concentration of Rhein in Plasma by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a RP-HPLC method for determining rhein in plasma.METHODS:ColumnSpherisorb C18(4.0mm?250mm,5?m),Mobile phase0.1% of phosphoric acid(pH 3.0)-methanol(30∶70,V/V),Flow rate1.0ml/min;Detection wavelength435nm.RESULTS:Rhein was completely separated and no interference was found.The peak areas of rhein within the range between 0.032～4.8?g/ml showed good linearity of the concentrations,r=0.9 990.The detection limit of rhein in plasma was 16ng/ml.The recovery rate was 92.09%～98.53%.The within-day RSD was 1.65%～6.68%,and between-day RSD was 4.93%～10.01%.CONCLUSION:This method is simple and sensitive,and can be used for analysing rhein-containing biological samples.