Effect of IL-12 on the expression of Fas/FasL and TNF? / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Artigo
em Chinês
| WPRIM
| ID: wpr-519102
ABSTRACT
AIM:
To study the effect of IL-12 on T lymphocytes apoptosis, the expression of Fas/FasL and TNFR/TNF?.METHODS:
Terminal dUTP nick end labeling(TUNEL) and Annexin V assay were used. Anti-TNFR were labeled with FITC, anti-CD95 was labeled with PE and Anti-FasL with biotin. Three kinds of T cells (HTB176,TIB152 and human normal T cells) were analysed through flow cytometry.RESULTS:
At 1st hour after being treated with IL-12, the expression of FasL protein and FasL mRNA in HTB176 and TIB152 began to increase and reached peak value in 24 hours. In the normal T cells, FasL just began to increase in 1 hour and maintained stability in 6, 12 and 24 hours through the later experiment period. All three kinds of T cells displayed no change in the expression of CD95 and TNFR/TNF? under the stimulation of IL-12.CONCLUSION:
Expression of such apoptosis regulating factors were different in the apoptosis of T cells induced by IL-12.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1989
Tipo de documento:
Artigo
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