Determination of Cefetamet in Dog Serum by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:A RP-HPLC method was developed for determination of cefetamet in serum METHODS:The serum samples were detected on a hypersil BDS C18 column after preciptating protein with methyl alcohol and being centrifuged at a speed of 10 000 r/min The mobile phase was methyl alcohol-water=28∶72(V/V),pH=2 8,the flow rate was 1 0ml/min and the detection wavelength 262nm RESULTS:The methods showed good linearity in a concentration range of 0 53～13 25?g/ml The minimun detactable concentration of cefetamet in serum was 0 25?g/ml,the mean recovery was 100 84%,the within-day RSD was less than 4 42% and the between-day RSD was less than 4 17% CONCLUSION:The method is simple,sensitive and accurate,and may be applied to the pharmacokinetics study of cefetamet in vivo