Determination of Methyl Hydroxybenzoate and Ethyl Hydroxybenzoate in Three Oral Liquid Preparations / 中国药房

ABSTRACT

OBJECTIVE:To establish the RP-HPLC method for determination of methyl hydroxybenzoate and ethyl hydroxybenzoate in oral liquid preparations METHODS:The Nova-Park C18 column(3 9mm?150mm,5?m)was used The mobile phase consisted of acetonitrile-water(25∶75)at a flow rate of 1ml/min,the detection wavelength was set at 254nm and the sensitivity was set at 0 01 AUFS RESULTS:The linear ranges of methyl hydroxybenzoate and ethyl hydroxybenzoate were 0 4 412～13 2 360?g/ml(r=0 9 999) and 0 6 672～20 0 160?g/ml(r=0 9 998)respectively CONCLUSION:The method is simple,rapid and accurate It can be used to detect antiseptics in hospital preparations