The Time Window of Myocardiocytes Apoptosis and Expression of Related Genes in Sinoaortic - denervated Rats / 中国药房

ABSTRACT

OBJECTIVE: To study the time window of apoptosis and related genes expression of the myocardiocytes in sinoaortic-denervated(SAD) rats.METHODS SAD of shamoperation(Sham) was performed in male SD rats at the age of 10 weeks.After 4, 8, 16, 32 weeks, apoptotic cells were stained in situ using terminal dexynucleotidyl - transferase mediated -dUTP nick end labeling(TUNEL) .All stained results were analysed by computer image analysis techniques.Bel-2, Bax, Fas and Fas-L were detected using immunohistochemical method.RESULTS: Compared with Sham controls, the number of apoptotic myocardiocytes was significantly increased;The expressions of Bel - 2 were significantly decreased, whereas Bax,Fas and Fas-L were significantly increased in SAD rats.The ratio of Bcl-2/ Bax was decreased.CONCLUSION Apoptosis and dysregulation of related gene expressions may be involved in the myocardial remodeling in SAD rats.