Effect of VEGF over-expressed C6 glioma cells on the expression of Flk-1 and Flt-1 in cocultured microvascular endothelial cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-519967
ABSTRACT
AIM:
To study the effects of VEGF over-expressed C6 glioma cells on the expression of Flk-1 and Flt-1 in cocultured microvascular endothelial cells using the rat hepatic cells BRL 3A as control.METHODS:
Cocultured systems of rat pulmonary microvascular endothelial cells with C6 and endothelial cells with BRL 3A were established. Immunocytochemical method was used to investigate the expression change of Flk-1 and Flt-1 protein in cocultured microvascular endothelial cells. The expression of Flt-1 and Flk-1 mRNA was analyzed by RT-PCR and Northern blot.RESULTS:
The microvascular endothelial cells cocultured with C6 showed increased expression of Flk-1 and Flt-1 protein ( P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
2000
Tipo de documento:
Artigo
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