Rapid Identification of Rickettsiae using the Real-Time PCR
Journal of Bacteriology and Virology
;
: 221-226, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-52015
ABSTRACT
In this study, new real-time PCR method based on the groEL gene was developed and investigated. Four spotted fever group (SFG) strains, four typhus group (TG) strains, and four scrub typhus group (STG) strains were easily differentiated as a distinct entity. This PCR assay was applied to detect Rickettsia DNA from 100 ticks. Twelve Haemaphysalis longicornis ticks were found positive and identified as spotted fever group Rickettsia. This real-time PCR method could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of SFG, TG, and STG in a single reaction.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Rickettsia
/
Carrapatos
/
DNA
/
Tifo por Ácaros
/
Tifo Epidêmico Transmitido por Piolhos
/
Reação em Cadeia da Polimerase
/
Diagnóstico Diferencial
/
Febre
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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