Determination of the Content of Baicaline in Qingkailing Injection With RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a RP-HPLC method for determination of the content of baicaline in Qingkailing Injection,so as to control the quality of this preparation.METHODS:The column was C18.The mobile phase was methanol-acetic acid-water(45∶1∶55).The detection wavelength was 274nm.RESULTS:There was a good linear relationship between the concentration of baicaline and absorption area value in range of 0.25～2.5?g/ml,r=0.9 992.The average recovery was 98.4%(n=5) with RSD=1.5%(n=5).CONCLUSION:The method was highly repeatable and accurate.