The effect of epidermal growth factor on graft small bowel morphology and absorption in rats / 中国普通外科杂志

ABSTRACT

Objective To study the effect of epidermal growth factor(EGF) on the morphology and absorption of small bowel graft. Method Total small bowel transplantion was performed between inbred Wistar(RT1 k) rats. Total parenteral nutrition(TPN group,n=10) and EGF supplemented TPN(EGF group,n=10) was used from POD2 to POD10. ResultsMucosal villous height,crypt depth, mucosal thickness and villous surface area were found significantly larger in EGF group than in TPN group.Normal ultrastructure of the graft was maintained in EGF group, Atrophied microvilli and broken mitochondrial crista were observed in TPN group. Plasma 15N-Glycine level was higher in EGF group than in TPN group. Conclusion EGF improves mucosal morphology,preserves enterocyte ultrastructure, enhances amino acid absorption.