Determination of the Content of Hydroxyproline in Liver Tissue by RP-HPLC in Rats with Hepatic Fi?brosis / 中国药房
China Pharmacy
;
(12)1991.
Artigo
em Chinês
| WPRIM
| ID: wpr-520479
ABSTRACT
OBJECTIVE:
To determine the content of hydroxyproline in liver tissue in rats with hepatic fibrosis.METHO_ DSRP-HPLC was used with precolumn derivation by2,4-dinitrochlorobenzene(DNCB)in the Borax buffer solution for lh in boiling water.Acetonitrile-acetate buffer linear gradient elution was used.Detecting wavelength was360nm.RESULTS:
Derivation of hydroxyproline could be separated from other amino acids and accurately determined.CONCLUSION:
The method is rapid,accurate,and suitable for detection of biopsy samples obtained by liver puncture.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
1991
Tipo de documento:
Artigo
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