Effects of bFGF and insulin on proliferation of mice cartilage tissue cells / 中国病理生理杂志

ABSTRACT

AIM: To explore the effects of basic fibroblast growth factor ( bFGF) and insulin on the cell proliferation and differentiation in primary cartilage cells. METHODS: After induction with different concentrations of bFGF and insulin, cell proliferation was measured with WST-1 method and fluoroscope methods. RESULTS: bFGF and insulin exerted their related action on primary cartilage cells in 0.4% fatal bovine serum at different concentrations. 25 ?g/L bFGF and 5 mg/L insulin promoted cell proliferation significantly. CONCLUSION: bFGF and insulin prolong the survival time and promote cell proliferation in primary cartilage cells.