Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Artigo
em Chinês
| WPRIM
| ID: wpr-520521
ABSTRACT
AIM:
To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro.METHODS:
GMC proliferation was detected by MTT method, GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis. The levels of Fas and Bcl-2 were also detected by immunohistology.RESULTS:
The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased.CONCLUSION:
CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS