Effect of Nicotine on the Growth of Rabbit Tendon Cells / 中国医师杂志

ABSTRACT

Objective To discuss the effect of nicotine on the growth of rabbit tendon cells.Method 1% concetration Nicotine(0 5ml/kg) was injected into rabbit's auditive vein,then femoral vein blood was extracted at 3 minutes later. Separating blood serum and add it into F-12 medium and 10% fetal bull serum,penicillin,streptomycin,vitC which was then used to culture rabbit tendon cells, observating the time of cell number growth duplication of test group and drawn up its growth curve. The control group was treated as the test group except the rabbit was not injected by nicotine.Results The time of cell number growth duplication of test group was 9?2 1 days and the control group was 7?1 1 days,there was difference significatly between the two groups.Conclusion Nicotine can restrain the growth of rabbit tendon cells and enhance tendon's adhesiveness in post-operation.