Improvement of the model of small bowel transplantation in rats / 中国普通外科杂志

ABSTRACT

Objective To improve the surgical technique to establish a simple model of heterotopic segmental small bowel transplantation in rats. Methods The segmental intestine was harvested with "non-injury"technique and consisted of donor aortic segment with superior mesenteric artery and portal vein, after perfused in situ with lactated Ringer's solution, the graft was stored in 4℃ lactated Ringer's solution for about 1 h. The heterotopic segmental intestine transplantation was performed by end-to-side anastomosis of donor aorta to recipient aorta. The left kidney of the recipient was resected and the donor's portal vein was anastomosed to the recipient left renal vein by" cuff anastomosis". Results A total of 87 intestine transplantations were performed, 26 of them was regarded as formal experiment group.The average time for the arterial and venous anastomosis were 25～30min and 5 min.Among the 26 recipient rats,21 survived more than 3days.The average survival was 8.93?2.59 days, The longest survival was 14 days in this group. Conclusions It is indicated that the keys of success of small intestinal transplantation are as followsthe high quality graft,excellent anastomosis of blood vessels and adequate liquid supplement.