A study on the expression of MDR1 mRNA in human hepatocellular carcinoma / 中国普通外科杂志

ABSTRACT

Objective To investigate MDR1mRNA quantitative expression in patients with hepatocellular carcinoma( HCC) and its relation to pathologic features, chemotherapy sensitivity, metastasis and prognosis. Methods Total RNA extracted from 47 cases of HCC and 12 normal liver tissue were subjected to analysis of MDR1mRNA expression by fluoregenic probe quantitative RT-PCR(FQ-RT-PCR). Result The level of MDR1mRNA expression was 1.4?10 3～3.6?10 6copies/?g RNA .The positive rates observed in 72% of the tumor tissue was significantiy higher than in 51% of the adjacent liver tissue and in 42% of the normal liver tissue (P