Immunohistochemical study of mast cell tryptase / 中国法医学杂志

ABSTRACT

Objective Carry out the mast cell tryptase (MCT) granule count in throat, lung and small intestine mast cells in persons died of anaphylactic shock.Method Control group 10 cases died from severe brain injury. Research group15 cases died from anaphylactic shock(group A)and 8 cases died of amniotic fluid embolism (group B). H & E staining and immunohistochemical staining were employed and the MCT granule count performed on the throat, lung and small intestine tissue.Results There was marked increase of MCT granule count in the throat (average MCT-positive number of group A 28.48 and of group B 24.35),lung (average MCT-positive number of group A 46.98 and of group B 43.50) and small intestine (average MCT-positive number of group A 48.23 and of group B 42.72) in specimens of group A and group B as compared with the control group (average number in throat 7.97,lung 12.94 and small intestine 20.25).Conclusion Tryptase granular count of MCT increased in throat, lung and small intestine tissue in persons died from anaphylactic shock and amniotic fluid embolism.